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KMID : 0604520180440020171
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Journal of the Society of Cosmetic Scientists of Korea
2018 Volume.44 No. 2 p.171 ~ p.181
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Effects of Anti-inflammation and Skin Barrier by Genistein Cyclodextrin Complex
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Choi Dong-Jun
Cho Uk-Min
Choi Da-Hee
Hwang Hyeon-Shik
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Abstract
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Genistein is one of the representative isoflavone compounds isolated from soybeans and has been studied very well for its anti-aging and anti-inflammatory activity through previous studies. However, although genistein exhibits high solubility in organic solvents, it shows low bioavaility due to the low water solubility. In this study, we compared directly the functional difference between genistein and genistein cyclodextrin complex which has the improved water solubility and stability by cell based assay. Cell cytotoxicity experiment were carried out on RAW264.7 with CCK-8 assay and cytotoxicity was appeared from 10 μ g/mL, thereby maximum concentration was set to 10 μ g/mL in all condition. We discovered that genistein CD complex suppressed NO production and iNOS expression as concentration dependent manner in the condition of LPS rather than genistein. Also, we could understand that genistein CD complex was able to down-regulate mRNA expression of anti-inflammatory cytokines such as IL1-α, IL1-β, IL-6, and TNF-α as concentration dependent manner in the presence of LPS. In addition, we verified that genistein CD complex increased TEER of HaCaT human keratinocyte cells as concentration dependent pattern and stimulated cell division and migration rather than genistein in cell migration assay. Thus, it is expected that it can be used as an effective cosmetic raw material for improving atopic dermatitis or skin barrier if clinical studies on skin regeneration and skin barrier of the genistein CD complex are carried out.
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KEYWORD
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genistein cyclodextrin complex, isoflavone, anti-inflammatory effects, cell migration, skin barrier
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